Principles of genomics and proteomics (Elsevier - Health Sciences Division)

Principles of Genomics and Proteomics: A Laboratory Guide is the perfect reference for graduate students and researchers in these areas to understand its principles and execute precise and reproducible experiments. Following an introductory chapter, the book dives into proper research, including genome mapping. The following sections cover in detail, following a detailed template, genomic and proteomic assays. Experiments present in the book span from Sangers Sequencing, Shotgun sequencing, SAGE analysis, DNA footprinting, Gel retardation, ChIP, moving then to protein resolution methods including PAGE, 2D gel electrophoresis and isoelectric focusing. Biophysical techniques are described in detail, including ultraviolet and visible light spectroscopy, fluorescence spectroscopy, NMR and X-ray diffraction. A final proteome analysis is dedicated to functional analysis. The final chapter covers applications of omics technologies broadly. Principles of Genomics and Proteomics: A Laboratory Guide is the perfect reference for genetics labs around the world. Graduate students will benefit from the structured and detailed coverage of methods. Established researchers can benefit from the book for staff training in research and may find it particularly helpful in enhancing reproducibility of experiments. Provides a comprehensive account of the technical aspects of genomic and proteomic analysis and their application to living organisms Detailed and standardized coverage of basic research methods in the area are provided Advanced applications of genomics and proteomics are highlighted with major significance in medical and agricultural fields